Integrity™ iCELLis™
Integrity™ iCELLis™, the world's first high-cell density bioreactor is designed to simplify your process, by combining the advantages of single-use technologies with the benefits of a fixed-bed system. Thanks to its compact and fully integrated design - for a quick implementation and more ease of use - iCELLis™ represents the new generation single-use bioreactors.
Central to the iCELLis™ bioreactor technology is the use of a compact fixed-bed, packed with custom macrocarriers. This matrix is made-up of medical grade polyester microfibers and provides up to 1,000 m² growth surface area in a compact bioreactor volume. By immobilizing the cells on this cell-friendly growth support, volumetric yields can be dramatically increased. Depending on cell lines and processes, a 20- to 100- fold increase in volumetric productivity - compared to stirred tank bioreactors - can easily be achieved.
iCELLis™ bioreactors are supplied pre-packed with macrocarriers. Compared to standard stirred tank bioreactor filled with µbeads, the iCELLis™ technology enables to avoid delicate and time consuming manual operations: sterilization and hydration of µbeads, and bead-to-bead transfers from preculture to final process.
With the biomass multiplication occurring in the fixed-bed, iCELLis™ bioreactors can be inoculated at very low density. The number and volumes of seed trains are reduced considerably: 2 CF40 (2.5 m²) or 6 Roller Bottles (1750 cm²) are sufficient to inoculate an iCELLis™ 1000 bioreactor. As a consequence of this process simplification, manual operations and associated costs are reduced dramatically.
Evenly-distributed media circulation is operated by a built-in magnetic drive impeller, ensuring low shear stress and higher cell viability. The cell culture medium flows throughout the fixed-bed from the bottom to the top. When at the top, the liquid falls as a thin film down the outer wall where it takes up O2 to oxygenate the cells in the bioreactor. This unique waterfall oxygenation, together with a gentle agitation and biomass immobilization, enables compact iCELLis™ to achieve and maintain high-cell densities, equaling the productivity of much larger stirred tank units.
Process simplification, from vial thawing to final product, is at the heart of the iCELLis platform. As cells are immobilized in the fixed-bed and iCELLis™ operates in perfusion mode, there is no need for centrifugation to harvest the cells, which simplifies extremely the downstream process. Last but not least, in order to reduce process development time, the perfusion device is integrated into the iCELLis™ skid.
iCELLis™ fixed-bed bioreactors are available in the following formats:
- iCELLIs™ nano for R&D application and quick evaluation – from 1 to 7 m² surface area
- iCELLis™ 200 for manufacturing – up to 200 m² surface area
- iCELLis™ 1000 for manufacturing – up to 1000 m² surface area
From bench to batch, choose iCELLis™.
Contact us to learn more on how the iCELLis™ platform can improve and simplify your process.
- Process simplification, from pre culture steps to final product:
- Inoculation at very low cell density, simplifying seed trains operations,
- Fixed-bed design avoids numerous operations involved by the use of µbeads,
- iCELLis™ bioreactors operate in perfusion mode simplifying DSP.
- Straightforward implementation:
- Standard process designed for quick implementation (less than one year),
- Linear scalability, from R&D scale to manufacturing scale,
- Integrated perfusion device - no additional equipment,
- iCELLis™ units are supplied in sterile (gamma-irradiation) and ready-to-use.
- Compact high-cell-density fixed-bed bioreactor:
- 20- to 100-fold increase in volumetric productivity vs. conventional stirred tanks,
- Manufacturing scales reduction enabling portability and rapid deployment,
- Require smaller facilities and simplified infrastructures.
- Dramatic decrease of operations costs and investments.
- Integrated mixing system for evenly-distributed media circulation and low shear stress.
- Custom specialized carriers for adherent and suspension cell cultures.
- Unique waterfall media oxygenation for high oxygen tranfer.
- Disposable probes pre installed (biomass, pH, DO, T).
- Single-use bioreactor made up USP Class VI rigid plastic to ensure process reliability.
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